منابع مشابه
Translocation of Vibrio harveyi N,N'-diacetylchitobiase to the outer membrane of Escherichia coli.
The gene encoding N,N'-diacetylchitobiase (chitobiase) of the chitinolytic marine bacterium Vibrio harveyi has been isolated. While expression of the chitobiase gene (chb) was inducible by N,N'-diacetylchitobiose in V. harveyi, it was expressed constitutively when cloned in Escherichia coli, suggesting that controlling elements are not closely linked to chb. Chitobiase was found in the membrane...
متن کاملThe quorum-sensing hybrid histidine kinase LuxN of Vibrio harveyi contains a periplasmically located N terminus.
Hydropathy profile analyses of the amino acid sequence of the quorum-sensing hybrid histidine kinase LuxN of Vibrio harveyi predict a periplasmic location of the N terminus. To test this, two-hybrid proteins consisting of LuxN and an N-terminally fused maltose-binding protein with or without a leader sequence were analyzed with regard to the enzymatic activities of LuxN, protease accessibility,...
متن کاملProbing the Catalytic Mechanism of Vibrio harveyi GH20 β-N-Acetylglucosaminidase by Chemical Rescue
BACKGROUND Vibrio harveyi GH20 β-N-acetylglucosaminidase (VhGlcNAcase) is a chitinolytic enzyme responsible for the successive degradation of chitin fragments to GlcNAc monomers, activating the onset of the chitin catabolic cascade in marine Vibrios. METHODS Two invariant acidic pairs (Asp303-Asp304 and Asp437-Glu438) of VhGlcNAcase were mutated using a site-directed mutagenesis strategy. The...
متن کاملMNDO Study of Nitrogen Atom Inversion in Piperazine, N,N'-Dimethylpiperazine and N,N'-Dichloropiperazine
MNDO semi-empirical SCF MO calculations are used to study the pyramidal nitrogen atom inversion and configurational equilibria in the title compounds.
متن کاملA Universal Investigation of $n$-representations of $n$-quivers
noindent We have two goals in this paper. First, we investigate and construct cofree coalgebras over $n$-representations of quivers, limits and colimits of $n$-representations of quivers, and limits and colimits of coalgebras in the monoidal categories of $n$-representations of quivers. Second, for any given quivers $mathit{Q}_1$,$mathit{Q}_2$,..., $mathit{Q}_n$, we construct a new quiver $math...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)63767-6